effect of different environments in homemade germination chambers on the germination and growth of cacao (Theobroma Cacao L.) rootstock in the Lodana parish of Santa Ana canton
DOI:
https://doi.org/10.59169/pentaciencias.v8i3.1893Keywords:
Germination; Mortality; BiomassAbstract
The objective of this research was to evaluate the effect of thermotherapy on the germination and development of cacao rootstocks (Theobroma cacao L.). The experiment was conducted using a completely randomized design (CRD) with five treatments: (T1) Germination chamber with transparent plastic, (T2) Germination chamber with perforated transparent plastic, (T3) Germination chamber with transparent plastic and zaram shade, (T4) Germination chamber with dark plastic, and (T5) Traditional germination (control). The evaluated variables included: days to germination, seed germination rate (%), seed mortality rate (%), seedling height (cm), stem diameter (mm), vigor or slenderness index, root length (cm), fresh aerial biomass (g), fresh root biomass (g), dry aerial biomass (g), dry root biomass (g), and Dickson’s quality index (DQI). Results showed that T1 achieved the fastest emergence (6 days), followed by T2 and T3 (8 days), while T5 required 9 days. Germination percentage exceeded 97% in T1 and T3, with minimal mortality (≤ 2%), compared to 96.55% germination and 3.45% mortality in T5. Regarding growth, T1 and T3 produced taller seedlings (41.5 cm) with higher slenderness indices; however, no significant differences were observed in stem diameter among treatments. T2 promoted the greatest root length (38.2 cm), whereas T1 generated the highest total biomass and the highest DQI (~0.73), indicating balanced and vigorous growth. The use of transparent plastic chambers, particularly when combined with zaram shading or perforations for ventilation, accelerates germination and improves the quality of cacao seedlings.
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Arévalo, G. E., Farfán, A., Barraza, F., Arévalo, H. C. O., Zúñiga, C. L. B., Alegre, J., & Baligar, V. C. (2021). Growth, physiological, nutrient-uptake-efficiency and shade-tolerance responses of cacao genotypes under different shades. Agronomy, 11(8). https://doi.org/10.3390/agronomy11081536
Canggih, N. M., & Malik, A. (2024). Seed viability and seedling growth of sulawesi 01 and iccri 08 cacao plant clones (Theobroma cacao L.). Jurnal Agroqua, 22, 293–299. https://doi.org/10.32663/ja.v21i2.4916
de Sousa, W. N., Brito, N. F., Felsemburgh, C. A., Vieira, T. A., & Lustosa, D. C. (2021). Evaluation of Trichoderma spp. Isolates in Cocoa Seed Treatment and Seedling Production. Plants, 10(9), 1964. https://doi.org/10.3390/plants10091964
Delgadillo, D, P., Soto, S, M., Rodriguez,P, L., Carrero, G, M., Torres, R, E., & Yockteng, R. (2020). A new method for the inoculation of Phytophthora palmivora (Butler) into cacao seedlings under greenhouse conditions. Plant Methods, 16(1). https://doi.org/10.1186/s13007-020-00656-8
Ding, D., Li, T., Wu, L., Zhang, X., Zhao, Y., Feng, H., Zhang, C., & Wendroth, O. (2024). Energy Compensation for Crop Growth under Plastic Mulching: Theories, Models, and Limitations. Agronomy, 14(5), 1005. https://doi.org/10.3390/agronomy14051005
ESPAC. (2024). INEC. Superficie cosechada y producción de plátano. https://www.ecuadorencifras.gob.ec/estadisticas-agropecuarias-2/
Harni, R., Amaría, W., Ferry, Y., & Marhaeni, L. S. (2020). Effect of Trichoderma spp. and potassium fertilizer on Phytophthora palmivora infection in cacao seedlings. IOP Conference Series: Earth and Environmental Science, 418(1), 012015. https://doi.org/10.1088/1755-1315/418/1/012015
Hossain, M. E., Zhang, Z., Dong, W., Wang, S., Liu, M., Liu, E., & Mei, X. (2022). Plastic Film Mulching Improved Maize Yield, Water Use Efficiency, and N Use Efficiency under Dryland Farming System in Northeast China. Plants, 11(13). https://doi.org/10.3390/plants11131710
Huang, C., Lin, L., Chen, F., Wang, X., Shi, M., Chen, L., Yang, X., Dong, X., & Zhang, M. (2025). Shading Effects on the Growth and Physiology of Endangered Hopea hainanensis Merr. & Chun Seedlings. Forests, 16(7), 1193. https://doi.org/10.3390/f16071193
Lahive, F., Handley, L. R., Hadley, P., & Daymond, A. J. (2021). Climate Change Impacts on Cacao: Genotypic Variation in Responses of Mature Cacao to Elevated CO2 and Water Deficit. Agronomy, 11(5), 818. https://doi.org/10.3390/agronomy11050818
Li, Q., Zhang, L., He, J., Li, J., Zhang, H., Li, Y., Gu, Y., Luo, H., Lu, M., Lu, K., & Xiong, L. (2025). Effects of different shade treatments on Melaleuca seedling growth and physiological properties. BMC Plant Biology, 25(1). https://doi.org/10.1186/s12870-025-06218-1
Mensah, E. O., Asare, R., Vaast, P., Amoatey, C. A., Markussen, B., Owusu, K., Asitoakor, B. K., & Ræbild, A. (2022). Limited effects of shade on physiological performances of cocoa (Theobroma cacao L.) under elevated temperature. Environmental and Experimental Botany, 201, 104983. https://doi.org/10.1016/j.envexpbot.2022.104983
Nguyen, G. N., Lantzke, N., & van Burgel, A. (2022). Effects of Shade Nets on Microclimatic Conditions, Growth, Fruit Yield, and Quality of Eggplant (Solanum melongena L.): A Case Study in Carnarvon, Western Australia. Horticulturae, 8(8), 696. https://doi.org/10.3390/horticulturae8080696
Odoemelam, V., Nwaigbo, L., Anyim, A., Aneni, T., & Anozie, C. (2023). Germination and Early Growth Performance of Cocoa (Theobroma cacao L.) Seedlings in Planting Media OPEN ACCESS Trends in. Trends in Agricultural Sciences, 2, 274–280. https://doi.org/10.17311/tas.2023.274.280
Organización de las Naciones Unidas para la Alimentación y la Agricultura FAO. (2023). Datos estadísticos FAOSTAT. Recuperado el 12 de mayo de 2025 de: https://www.fao.org/faostat/en/#data/QCL.
Osorio, Z. M. A., Castillo, D. A., Rodríguez, P. L., & Terán, W. (2021). Cacao (Theobroma cacao L.) Response to Water Stress: Physiological Characterization and Antioxidant Gene Expression Profiling in Commercial Clones. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.700855
Reis, L. C., Scalon, S. P. Q., Foresti, A. C., Dresch, D. M., Santos, C. C., & Lima, V. T. (2023). How does shading mitigates the water deficit in young Hymenaea courbaril L. plants? Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1235234
Vásquez, C., Orozco, A., Sánchez, E., & Cervantes, V. (2020). La reproducción de las plantas: Semillas y Meristemos (en línea). Consultado el 28 mar. 2025.
Xue, G., Wu, J., Zhou, B., Zhu, X., Zeng, J., Ma, Y., Wang, Y., & Jia, H. (2023). Effects of Shading on the Growth and Photosynthetic Fluorescence Characteristics of Castanopsis hystrix Seedlings of Top Community-Building Species in Southern Subtropical China. Forests, 14(8), 1659. https://doi.org/10.3390/f14081659
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