Detection of Shigella spp. in samples of chicken meat that are spent in the Ambato canton
DOI:
https://doi.org/10.59169/pentaciencias.v5i5.745Keywords:
Shigella spp., chicken meat, isolation, molecular identification, Public Health.Abstract
Shigella spp. is the causative agent of gastric infections commonly acquired by the consumption of contaminated food. Chicken meat is an important transmission source of shigellosis due to its high demand and consumption. The purpose of this article is the detection of Shigella spp in chicken meat that is sold in Ambato from different markets. 135 samples were taken, 45 from places with an operating licence, 45 from informal food markets and 45 from slaughterhouses that supply the city of Ambato. Phenotypic identification was performed by culturing bacteria on selective medium, Gram staining and TSI. Shigella spp. candodates were sent for molecular identification by amplification of the 16S region of ribosomal DNA, where the presence of Shigella sonnei (10), Shigella flexneri (5) and Shigella dysenteriae (2) was demonstrated, whereas that Shigella boydii was not in any of the samples. In summary, three species of Shigella were identified, predominantly S. sonnei with greater inclination in slaughterhouses and informal outlets, which can mean a serious problem for Public Health.
Downloads
References
Al-Hasani, K., Henderson, I., & Sakellaris, H. (2000). The sigA gene which is borne on the she pathogenicity island of Shigella flexneri 2a encodes an exported cytopathic protease involved in intestinal fluid accumulation. Infection and Immunity, 68(5). doi:https://10.1128/IAI.68.5.2457-2463.2000
Bachir, F., Rafei, R., Osman, M., & et_al. (15 de september de 2021). Historical, current, and emerging tools for identification and serotyping of Shigella. doi:https://10.1007/s42770-021-00573-5.
Baker, Stephen; Chung, Hao. (06 de septiembre de 2018). Recent insights into Shigella: a major contributor to the global diarrhoeal disease burden. doi:https://10.1097/QCO.0000000000000475
Bani, M., Payel, M., & Moumita, D. (2021). Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1. Scientific Reports. doi:https://10.1038/s41598-021-98910-z
Bantawa, K., Rai, K., & Subba, D. (2018). Food borne bacterial pathogens in marketed raw meat of Dharan, eastern Nepal. BMC Research Notes. doi:https://10.1186/s13104-018-3722-x
Bing-Mu, Shu-Fen, Shih-Wei, & et_al. (2010). Differentiation and identification of Shigella spp. and enteroinvasive Escherichia coli in environmental waters by a molecular method and biochemical test. doi:https://doi.org/10.1016/j.watres.2009.10.004
Cetinkaya, F., Cibik, R., & Soyutemiz, E. (noviembre de 2008). Shigella and Salmonella contamination in various foodstuffs in Turkey. doi:https://doi.org/10.1016/j.foodcont.2007.11.004
Clermont, O., Christenson, J., & Gordon, D. (5 de febrero de 2013). The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. doi:https://10.1111/1758-2229.12019
Cortés-Sánchez, Espinosa-Chaurand, Garcia-Barrientos, & et_al. (abril de 2021). Fish, Tilapia, and Shigellosis. AGRICULTURAL RESEARCH, 17(4). doi:https://doi.org/10.5897/AJAR2021.15436
González-Corona. (2017). Diarrea aguda, prolongada y persistente en niños y su diferencia de la diarrea crónica. Obtenido de Scielo: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30192017000900012
Gosa, G. (s.f.). Antibiogram and Growth Potential of Salmonella and Shigella in Ethiopia: Implications for Public Health: A Review. Research Journal of Microbiology, 10(7), 288-307. doi:https://10.3923/jm.2015.288.307
Haileselassie, M., Taddele, H., Adhana, K., & et_al. (s.f.). Food safety knowledge and practices of abattoir and butchery shops and the microbial profile of meat in Mekelle City, Ethiopia. ELSEVIER, 3(5), 407-412. doi:https://10.1016/S2221-1691(13)60085-4
Henríquez, T., Salazar, J., Marvasi, M., & et_al. (24 de enero de 2020). SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000. PLOS ONE. doi:https://doi.org/10.1371/journal.pone.0228178
Jun-Seob, Moo-Seung, & Ji-Hyung. (04 de junio de 2020). Recent Updates on Outbreaks of Shiga Toxin-Producing Escherichia coli and Its Potential Reservoirs. Frontiers in Cellular and Infection Microbiology , 10(273). doi:https://doi.org/10.3389/fcimb.2020.00273
Lampel, K., Formal, S., & Maurelli, A. (08 de enero de 2018). A Brief History of Shigella. EcoSal Plus. doi:https://10.1128/ecosalplus.ESP-0006-2017
Levine, M., Kotloff, K., Nataro, J., & et_al. (2012). The Global Enteric Multicenter Study (GEMS). Clinical Infectious Diseases, 215-221. doi:https://10.1093/cid/cis761
Moposita-Molina. (2017). Moposita Molina, F.D. (2017) Identificación de bacterias, microalgas y hongos termófilos de la fuente geotermal “Chachimbiro” mediante técnicas microbiológicas y moleculares. [. Obtenido de Universidad de las Fuerzas Armadas: https://repositorio.espe.edu.ec/bitstream/21000/13127/1/T-ESPE-057258.pdf
Rahimi, E., Shirazi, F., & Khamesipour, F. (07 de enero de 2016). Isolation and Study of the Antibiotic Resistance Properties of Shigella Species in Meat and Meat Products. Journal of Food Processing and Preservation. doi:https://doi.org/10.1111/jfpp.12947
Rahman, A., Haque, A., Ahmad, T., & et_al. (01 de noviembre de 2019). Isolation, Identification and Antibiotic Sensitivity Pattern of Salmonella spp. ELSEVIER, 175-180. doi:https://doi.org/10.1016/j.micpath.2019.03.037
Ruiz, L., & Pons, M. (septiembre de 2018). Presencia de Enterobacteriaceae y Escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en Lima. Rev Peru Med Exp Salud Publica, 425-432. doi:https://doi:10.17843/rpmesp.2018.353.3737
Sakellaris, H., Luck, S., Al-Hasan, K., & et_al. (2004). Regulated site-specific recombination of the pathogenicity island of. Molecular Microbiology, 52(5), 1329–1336. doi:https://10.1111/j.1365-2958.2004.04048.x
Shahin, K., Zhang, L., & Soleimani, A. (junio de 2021). Effective control of Shigella contamination in different foods using a novel six-phage cocktail. doi:https://doi.org/10.1016/j.lwt.2021.111137
Toro, C., Salazar, J., Montero, D., & et_al. (04 de febrero de 2022). Antimicrobial Resistance Dynamics in Chilean Shigella sonnei Strains Within Two Decades: Role of Shigella Resistance Locus Pathogenicity Island and Class 1 and Class 2 Integrons. Frontiers in Microbiology , 12. doi:https://3389/fmicb.2021.794470
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Revista Científica Arbitrada Multidisciplinaria PENTACIENCIAS - ISSN 2806-5794.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
