Identificación molecular de la bacteria Escherichia coli en muestras de carne de pollo que se expenden en el cantón Ambato

María Elisa Barros Iza; Sandra  Cruz Quintana; Solange Escobar Aguilar; Jennifer Gómez Usiña; Carolina Manzanillas Miranda

doi:10.59169/pentaciencias.v5i5.770

Authors

María Elisa Barros Iza Universidad Técnica de Ambato https://orcid.org/0009-0000-8831-9398
Sandra Cruz Quintana Universidad Técnica de Ambato https://orcid.org/0000-0002-8510-1294
Solange Escobar Aguilar Universidad Técnica de Ambato https://orcid.org/0009-0005-5864-9034
Jennifer Gómez Usiña Universidad Técnica de Ambato https://orcid.org/0009-0002-7157-6739
Carolina Manzanillas Miranda Universidad Técnica de Ambato https://orcid.org/0009-0007-9956-3784

DOI:

https://doi.org/10.59169/pentaciencias.v5i5.770

Keywords:

chicken meat; strains; contamination; Escherichia coli; 16S rRNA gene.

Abstract

Chicken meat is one of the most consumed proteins worldwide, however, it is one of the main reservoirs of Escherichia coli which is the main cause of multiple infections after consumption due to different factors such as inefficient handling at the time of slaughter, poor hygiene and inadequate refrigeration. The diseases it causes not only affect the health of consumers, but also the economy of traders. Therefore, this study aimed to identify by sequencing the 16S rRNA gene different strains that were isolated in chicken meat, for this 135 samples were taken and collected from formal and informal points of sale and from 9 slaughtering centers that supply Ambato. The samples were evaluated by means of microbiological and biochemical tests and then DNA was extracted from the 40 suspect samples, which were analyzed by PCR. The results were obtained through sequencing where a total of 33 different strains were identified, strains AF1 and ATCC 11775 were predominant in the chicken meat samples in the canton of Ambato.

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References

Barel , M., Hizlisoy, H., Gungor , C., & et al. (2022). Escherichia coli serogroups in slaughterhouses: Antibiotic susceptibility and molecular typing of isolates. Int J Food Microbiol 16;371:109673.

Bastidas León, A. (2018). Determinación de Escherichia coli O157: H7 por el método Oficial AOAC 996.09 en carne de res faenada. Tesis de Química de Alimentos.Quito:Univ.Central del Ecuador.107p.

Feye, K., Thompson , D., Rothrock , M., Kogut , M., & Ricke , S. (2020). Poultry processing and the application of microbiome mapping. Poult Sci 99(2):678-688.

Jang, J., Hur, H. G., Sadowsky, M., Byappanahalli, M., Yan, T., & Ishii, S. (2017). Environmental Escherichia coli: ecology and public health implications. Applied Microbiology 123(3):570-581.

Jang-Seu Ki , Wen Zhang, & Pei-Yuan Qian. (2009). Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification. Journal of Microbiological Methods 77 (1): 48-57.

Koesoemo , D., Prihastuti, A., Elziyad , M., Ayu, D., & Annisa, S. (2021). Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia. Veterinary World 14 (12): 3138-3143.

Köhler, C., & Dobrindt , U. (2011). What defines extraintestinal pathogenic Escherichia coli? International Journal of Medical Microbiology 301(8):642-647.

Liaqat , Z., Khan , I., azam , s., Anwar, Y., Althubaiti , E., & maroof , l. (2022). Isolation and molecular characterization of extended spectrum beta lactamase producing Escherichia coli from chicken meat in Pakistan. PLOS ONE 17 (6): e0269194.

Lucas, J., Morales, S., Salazar, E., Eslava, C., & Alvarado, D. (2016). Contaminación por Escherichia coli Shigatoxigénica en Puestos de Expendio de Carne de Pollo en un Distrito de Lima. Revista de Investigaciones Veterinarias del Perú 27(3), 618–625.

Nguyen , T., Khong, D., le , h., Tran, H., Phan, Q., Le, H.,Yamamoto, Y. (2021). Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets. Biomed Res Int 2021: 6678901.

Pacholewicz, E., Swart , A., Wagenaar, J., Lipman, L., & Havelaar, A.(2016). Associated with Campylobacter and Escherichia coli Concentrations on Broiler Chicken Carcasses during Processing in Two Slaughterhouses. J Food Prot 79(12):2038-2047.

Pakbin, B., Bruck, W., & Rossen, J.(2021).Virulence Factors of Enteric Pathogenic Escherichia coli: A Review. . Int J Mol Sci 22(18):9922.

Pérez Pintado, A. C., & Astudillo Vallejo, K. F.(2020). Caracterización molecular de aislados de Escherichia coli en pollos de carne (Gallus gallus domesticus) de la provincia de Azuay, Ecuador.Tesis Médico Veterinario Zootecnista.41p.

Psonis, J., & Thanassi, D. (2019). Therapeutic Approaches Targeting the Assembly and Function of Chaperone-Usher Pili. EcoSal Plus. EcoSalPlus 8(2):10.1128/ecosalplus.ESP-0033-2018

Ramírez Aguilera, J., Medina Romero, Y., & Uscanga Garcia, I.(2018). MANUAL DE LABORATORIO DE MICROBIOLOGIA .Disponible en :https://www.uv.mx/qfb/files/2020/09/Guia-de-Microbiologia.pdf

Reyes, N., Talavera, M., Varela, J., Barba, J., Gutiérrez, A., & Alonso, U. (2013). Prevalencia y resistencia a antibióticos de Escherichia coli O157:H7 aislada de canales de bovinos sacrificados en rastros del altiplano central Mexicano. Revista Mexicana de Ciencias Pecuarias 4(2):235-242.

Riley, L.(2020). Distinguishing Pathovars from Nonpathovars: Escherichia coli. Microbiol Spectr 33385193

Salas, M., Benito , S., Pérez , A., del Amo Arregui, C., Ormazabal , M., & Hidalgo , M.(2022). Secuenciación de bacterias ARNR 16S. Revista Sanitaria de Investigación.Disponible en:https://revistasanitariadeinvestigacion.com/secuenciacion-de-bacterias-arnr-16s/.

Sebsibe, M. A., & Asfaw, E. T. (2020). Occurrence of Multi-Drug Resistant Escherichia Coli and Escherichia Coli O157:H7 in Meat and Swab Samples of Various Contact Surfaces at Abattoir and Butcher Shops in Jimma Town, Southwest District of Ethiopia. Infect Drug Resist 13:3853-3862.

Sivertsen, A., Dyrhovden, R., Gjerde , M., Sæle Bruvold, T., Nybakken , E., Skutlaberg , D., Kommedal , Ø. (2022). Escherichia marmotae-a Human Pathogen Easily Misidentified as Escherichia coli. Microbiol Spectr 10(2):e0203521.

Trokhymchuk, A., Waldner , C., gow, s., Chaban, B., & Colina, J.(2014). Comparison of baseline bacterial levels in retail ground beef originating from different regulatory, processing, and packaging environments. J Food Prot, 77(3):404-411.

Valenzuela, F., Casillas, R., Villalpando, E., & Vargas, F. (2015). El gen ARNr 16S en el estudio de comunidades microbianas marinas. Ciencias Marinas 41 (4), 297–313.

Vásquez Ampuero, J., & Tasayco Alcántara, W. R. (2020). Presencia de patógenos en carne cruda de pollo en centros de expendio, Huánuco-Perú: una problemática en salud. Revista de la Sociedad de Investigación Selva Andina vol. 11, núm. 2, 2020, pp. 130-141.

Zarei , O., Shokoohizadeh, L., Hossainpour, H., & Alikhani, M.(2021). The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples. International Journal of Microbiology 2021: 3333240.